ZION is a appears to work by causing uncontrolled cell division in vascular tissue. Abnormal increases in cell wall plasticity, biosynthesis of proteins, and production of ethylene occur in plant tissues following exposure, and these processes are responsible for uncontrolled cell division. The ester forms of 2, 4- D penetrate foliage, whereas plant roots absorb the salt forms. 2, 4-D appears to be similar in action to other auxin-type herbicides.

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